In vitro fertilization with flow-cytometrically-sorted bovine sperm

摘要: An attractive feature of IVF is that fewer sexed sperm are needed than for artificial insemination. However, by flow cytometry/cell sorting probably pre-capacitated, necessitating modifications to standard systems optimal success. With current procedures, the percentages oocytes fertilized with sorted and unsorted frozen bovine similar, events during first cell cycle timed similarly sperm. in most cases, blastocyst production was approximately 70% controls produced In some early studies, there appeared be an unexplained delay about half a day development. Nevertheless, dozens apparently normal calves, pre-sexed 90% accuracy, have resulted from embryos via also has proven useful as bioassay improving sperm-sorting procedures such determining potential detrimental effects laser power. It likely use cattle breeding programs will increase considerably when sexed, become commercially available.