Aberrant expression of tight junction-related proteins ZO-1, claudin-1 and occludin in synovial sarcoma: an immunohistochemical study with ultrastructural correlation.

摘要: Synovial sarcoma demonstrates epithelial differentiation, either by light microscopy (biphasic synovial sarcoma) or immunohistochemical/ultrastructural methods only (monophasic) and poorly differentiated sarcoma. Although the glands of are known to have tight junction-like structures, far less is about junction formation in spindled component sarcomas. Additionally, it unknown whether junctions normally constituted. The a multiprotein complex consisting numerous proteins that include ZO-1, claudin-1 occludin. A total 35 cases (13 biphasic, 14 monophasic eight differentiated) were immunostained for occludin using commercially available antibodies, heat-induced epitope retrieval standard avidin-biotin technique. When available, corresponding electron micrographs reviewed. For five cases, presence an SYT-SSX1 (three cases) SYT-SSX2 (two gene fusion was known. Positive showed particulate membrane staining. biphasic sarcomas expressed ZO-1 (13/13), (12/13) (11/13) manner identical normal glandular epithelia, at apical portion lateral membrane. spindle cells abnormal circumferential membranous expression (12/13), (6/13) (3/13). Monophasic pattern (13/14) but often (4/14) (3/14). Poorly (8/8) (6/8) rarely (2/8). By microscopy, recognizable seen glands. No correlation between histologic subtype type proteins. We conclude show well-organized, true junctions. In contrast, all significant abnormalities localization proteins, suggesting partial and/or aberrant differentiation.