In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus).

摘要: Tick-borne encephalitis is the most important tick-transmitted zoonotic virus infection in Eurasia, causing severe neurological symptoms humans. The causative agent, tick-borne (TBEV), circulates between ticks and a variety of mammalian hosts. To study interaction TBEV one its suspected reservoir hosts, bank voles Western evolutionary lineage were inoculated subcutaneously with either eight strains or related attenuated Langat virus, euthanized after 28 days. In addition, subset four was characterized Carpathian linage. Six per strain, housed together groups three uninfected in-contact animal each. Generally, did not show any clinical signs over course infection. However, infected vole died had to be prematurely, all which been identical strain (Battaune 17-H9, isolated 2017 Germany from vole). All animals seroconverted, while none did. Viral RNA detected via real-time RT-PCR whole blood samples 31 out 74 surviving voles. corresponding serum sample remained PCR-negative nearly cases (29/31). brain and/or spine tested positive 11 cases, mostly correlating sample. Our findings suggest good adaption voles, combining low virulence phenotype detectable replication hinting at host function for TBEV.