The Beneficial Effect of Delayed Compressive Loading on Tissue-Engineered Cartilage Constructs Cultured with TGF-β3

摘要: Summary Objective To determine whether the functional properties of tissue-engineered constructs cultured in a chemically-defined medium supplemented briefly with TGF-β3 can be enhanced application dynamic deformational loading. Methods Primary immature bovine cells (2–3 months old) were encapsulated agarose hydrogel (2%, 30×10 6 cells/ml) and for first 2 weeks transforming growth factor beta 3 (TGF-β3) (10μg/ml). Physiologic loading (1Hz, 3h/day, 10% unconfined deformation initially tapering to 2% peak-to-peak by day 42) was applied either concurrent or after period supplementation. Mechanical biochemical evaluated up 56. Results Dynamic concurrently supplementation yielded significantly lower (−90%) overall mechanical when compared free-swelling controls. In contrast, same protocol discontinuation resulted increased (+10%) relative Equilibrium modulus values reach 1306±79kPa glycosaminoglycan levels 8.7±1.6% w.w. during this 8-week are similar host cartilage (994±280kPa, 6.3±0.9% w.w.). Conclusions An optimal strategy tissue engineering articular cartilage, particularly accelerate construct development, may incorporate sequential different factors