Targeted p120-Catenin Ablation Disrupts Dental Enamel Development

摘要: Dental enamel development occurs in stages. The ameloblast cell layer is adjacent to, and responsible for, formation. When rodent pre-ameloblasts become tall columnar secretory-stage ameloblasts, they secrete matrix proteins, the ameloblasts start moving rows that slide by one another. This movement necessary to form characteristic decussating prism pattern. Thus, a dynamic system of intercellular interactions required for proper development. Cadherins are components adherens junction (AJ), span membrane mediate attachment cells. p120 stabilizes cadherins preventing their internalization degradation. So, we asked if p120-mediated cadherin stability important dental Targeted ablation mouse organ had striking effect. Secretory stage detached from surrounding tissues, lost polarity, flattened, E- N-cadherin expression became undetectable immunostaining. itself was poorly mineralized appeared be composed thin merged spheres abraded tooth. Significantly, mosaic teeth were capable forming normal demonstrating defects not secondary effect ablation. Surprisingly, blood-filled sinusoids developed random locations around developing teeth. has been observed other p120-ablated tissues may due altered signaling. These data reveal critical role tooth consistent with directing detachment secretory as move rows.