Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival.

摘要: Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) contribute to its resistance adjuvant treatment. MicroRNAs (miRNAs) small, non-coding RNAs controlling gene expression at post-transcriptional level, which known be important regulators features. Moreover, miRNAs have been previously proved promising diagnostic biomarkers in several cancers including GBM. Using global analysis 10 paired in-vitro as well in-vivo characterized primary GSC non-stem cultures, we identified a miRNA signature associated with phenotype 51 most deregulated classified cell cultures into clusters subgroup more pronounced stem-cell characteristics. The importance was further supported by demonstrating that Risk Score based on seven overexpressed predicted overall survival GBM patients TCGA dataset independently IDH1 status. In summary, differentially expressed GSCs described their association patient survival. We propose these participate features could represent helpful prognostic markers potential therapeutic targets