Genomic and Bioinformatic Characterization of Mouse Mast Cells (P815) Upon Different Influenza A Virus (H1N1, H5N1, and H7N2) Infections.

摘要: Influenza A virus (IAV) is a segmented negative-stranded RNA that brings potentially serious threat to public health and animal husbandry. Mast cells play an important role in both the inherent adaptive immune response. Previous studies have indicated mast support productive replication of H1N1, H5N1, H7N2. To date, distinct molecular mechanism behind pathogenesis among three different viruses has been poorly understood. In this study, we investigated genomic profiles detail dynamic change genomes regulated by subtypes IAV mouse using microassays. Compared with any two IAV-infected groups, many more differentially expressed genes (DEGs), cellular functions, signaling pathways were confirmed H1N1 or H7N2 group, group showing highest levels. However, few DEGs detected various functions dramatically suppressed H5N1 group. With in-depth study on demonstrated essential 5-HT pathway cyclic guanosine monophosphate (cGMP)/protein kinase G (PKG) pathway, which preferentially activated P815 infected crucial HIF-1 was virus. Furthermore, real-time quantitative polymerase chain reaction (RT-qPCR) results showed significantly increased mRNA levels PKG H1N1-infected H7N2-infected cells. addition, exosomes secreted from are pivotal innate immunity fight infection. This provides novel information insight into perspective profiles.