Inflammatory response during hyperglycemia and hyperinsulinemia in a porcine endotoxemic model: the contribution of essential organs.

摘要: Background:  During euglycemia acute hyperinsulinemia diminishes the cytokine response to endotoxin [Lipopolysaccharide (LPS)] exposure. In this study we elucidated whether hyperglycemia and modify content in several organs during LPS challenge a porcine model. Methods:  Pigs (35–40 kg) were randomized either normoglycemia (group 1, n = 8) or 2, n = 8), anesthetized mechanically ventilated. Both groups received 180-min intravenous infusion of (total 10 µg kg−1). Groups 1 2 clamped at plasma glucose concentrations 5 mM 15 mM, respectively. Group maintained baseline insulin level while hyperglycemic group exhibited increased levels. Results:  Circulating cytokines, mRNA protein examined heart, liver, kidneys, lungs, spleen, adipose muscle tissue. After exposure, both vast equal cytokines elicited by approximately 70–5000-fold. A 10-fold higher IL-10, IL-6 TNF-α was found kidney tissue compared other together with 3–10-fold increase However, mRNAs as well organ function without statistical difference between groups. Conclusion:  Endotoxemia pronounced level. The kidneys showed highest content. Acute apparently counteracts well-established anti-inflammatory effects on inflammatory challenged model. Whether observation can be extrapolated more long-term stress-exposure remains clarified.