Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells.

摘要: Neural precursor cells (NPCs) are lineage-restricted neural stem with limited self-renewal, giving rise to a broad range of cell types such as neurons, astrocytes, and oligodendrocytes. Despite this developmental potential, the differentiation capacity NPCs has been controversially discussed concerning trespassing lineage boundaries, for instance resulting in hematopoietic competence. Assessing their vitro plasticity, we isolated nestin+/Sox2+, from adult murine hippocampus. In vitro-expanded were able form neurospheres, self-renew, differentiate into neuronal, astrocytic, oligodendrocytic cells. Although cultivated early passage efficiently gave neuronal directed assay, extensively revealed reduced potential ectodermal differentiation. We further observed successful long-term cultured osteogenic adipogenic types, suggesting that underwent fate switch during culture. more than 12 passages aneuploid (abnormal chromosome numbers 70 chromosomes). Furthermore, they showed growth factor-independent proliferation, hallmark tumorigenic transformation. conclusion, our findings substantiate restriction mammalian Prolonged cultivation results, however, enhanced which may be attributed transformation events leading