Evaluation of canine-specific minocycline and doxycycline susceptibility breakpoints for meticillin-resistant Staphylococcus pseudintermedius isolates from dogs.

摘要: Background Using the US Clinical and Laboratory Standards Institute (CLSI) human tetracycline breakpoints to predict minocycline doxycycline susceptibility of Staphylococcus pseudintermedius (SP) isolates from dogs is not appropriate because they are too high meet pharmacokinetic/pharmacodynamic data using a standard dose. New have been approved for proposed minocycline. Revised four dilutions lower than breakpoints, providing more conservative classification isolates. Hypothesis/Objectives The objectives this study were measure minimum inhibitory concentrations (MICs) 100 canine meticillin-resistant SP clinical isolates, compare their susceptibilities based on current revised standards, document resistance genes. Methods E-test strips used determine MICs. PCR was identify tet genes. Results Using breakpoint MIC ≤ 4 μg/mL, 76 susceptible 36 doxycycline. In contrast, (MIC ≤ 0.25 μg/mL) (MIC ≤ 0.125 μg/mL), 31 both Thirty-one carried no genes, two had tet(K) 67 tet(M). Conclusions importance Use misclassified 45 five as doxycycline, respectively. analysis revealed that 43 classified respectively, possessed gene, tet(M), known confer drugs. These results underscore importance utilizing in place breakpoints.