Glycosphingolipids in detergent-insoluble substrate attachment matrix (DISAM) prepared from substrate attachment material (SAM)

摘要: Abstract The glycosphingolipids isolated from the detergent-insoluble material (DIM) of whole cells as well a similar substrate attachment matrix (DISAM) have been investigated in comparison with cells. proportion glycolipids total lipid extract was enriched DISAM DIM fractions compared to ratio ganglioside (GM3) neutral also higher than radioactivity incorporated into BHK cells, metabolically labeled radioactive glucosamine, greater confluent sparsely growing cells; however, label incorporation fraction BHKpy 2–3-fold that although chemical quantity GM3 much lower In order confirm enhanced by other procedures, were detached EGTA, washed, and reattached on plates. amount Cell spreading cell plastic plate inhibited inclusion medium. These data suggest that: (i) glycolipids, particularly GM3, at site different metabolic activity those goes preferentially sites, may some functional role regulating (ii) turnover sites are actively yet transformed any state non-transformed