The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron.
作者： Biljana Buzadzic , Vesna Otasevic , Ksenija Velickovic , Aleksandra Jankovic , Milica Markelic
DOI: 10.14670/HH-28.493
关键词:
摘要: The aim of this study was to investigate lipofuscin origin in brown adipocytes hyper-insulinaemic rats and the possible role lipid peroxidation iron process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement production, accompanied by an increase mitochondrial damage adipocytes. Extensive fusions droplets mitochondria with lysosomes were also observed. Confocal microscopy showed auto-fluorescence emission adipose tissue (BAT) after excitation at 488 nm 633 nm, particularly insulin-treated groups. presence distribution product, 4-hydroxy-2-nonenal (4-HNE), assessed immunohistochemical revealing its higher content treatment insulin. quantified electron dispersive X-ray analysis (EDX) showing ultrastucture majority granules suggests their origin, which additionally confirmed co-localization ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis hyperinsulinaemic is a consequence peroxidation, accumulation
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