TFF3 mediated induction of VEGF via hypoxia in human gastric cancer SGC-7901 cells

摘要: Increasing evidence indicates that in gastric epithelial cells, induction of TFF3 by hypoxia is mediated HIF-1. Since VEGF one the most important angiogenic factors on cancer progression, we have started to investigate possible link among HIF-1α, VEGF, and cells. We induced hypoxic condition SGC-7901cells using hypoxia-mimetic agent CoCI2. SGC7901 cells were transfected with pcPUR + U6 plasmid carrying RNAi targeted human selected puromycin-resistant pools establish stable knockdown Our results showed HIF-1a via consequences increased expressions SGC-7901 Overexpression upregulated mRNA hypoxia, impaired upregulations hypoxia. Furthermore, reduced protein secretion: as secretion was time dependent manner response TFF3-WT cells; however, production significantly decreased TFF3-KD (621 ± 89 vs. 264 ± 73 at 6 h 969 ± 97 508 ± 69 12 h, P < 0.05). data demonstrated regulation expression implicated might be applied a potential anti-angiogenic target for treatment cancer.