Determination of aminoglycoside residues by liquid chromatography and tandem mass spectrometry in a variety of matrices

摘要: Abstract A quantitative LC–MS/MS method was developed for the determination of 13 commonly used aminoglycoside antibiotics in meat (pork muscle, fish, and veal livers kidneys). The proposed is sufficiently sensitive highly selective. Unlike other previously reported methods, it uses a simple clean-up procedure based on strong cation-exchange solid-phase cartridge that permits high sample extract loading volumes. unique elution regime volatile buffer at intermediately pH value combination with an organic solvent provides various aminoglycosides. This methodology ensured neither breakthrough weakly retained aminoglycosides (e.g. spectinomycin) nor incomplete strongly analytes neo- gentamycin) observed. single-step fast produces clean extracts minimize matrix-related signal suppression electrospray interface.