Overexpression of Cyclooxygenase-2 in Carcinoma of the Pancreas
作者： Koji Umeshita , Morito Monden , Masato Sakon , Shingo Noura , Osamu Ishikawa
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摘要: The level of cyclooxygenase (COX)-2 has been investigated recently in various human carcinomas. In the present study, we examined distribution and extent COX-2 protein pancreatic tumors using immunohistochemistry. A strong expression was 23 52 (44%) carcinomas, a moderate 24 (46%) weak 5 (10%) contrast, benign showed or no COX-2, only islet cells displayed normal tissues. Overexpression carcinoma tissues also confirmed by Western blot analysis. Furthermore, consistent with results at levels, reverse transcription-PCR analyses indicated that mRNA overexpressed 7 13 (54%) but none 3 tumors. Our findings suggest inhibitors might be potentially effective against carcinomas may involved certain biological processes islets.
aacrjournals.org参考文章(53)
J Finke, W Lange, G Dölken, P Ternes, R Fritzen, An improved strategy and a useful housekeeping gene for RNA analysis from formalin-fixed, paraffin-embedded tissues by PCR. BioTechniques. ,vol. 14, pp. 448- 453 ,(1993)
Paola Capelli, S. Hirohashi, Aldo Scarpa, Giuseppe Zamboni, Calogero Iacono, T. Oda, K. Mukai, Pancreatic adenocarcinomas frequently show p53 gene mutations American Journal of Pathology. ,vol. 142, pp. 1534- 1543 ,(1993)
Shinichi Kitada, Maryla Krajewska, Jurgita Sauvageot, Ahmed Shabaik, Stanislaw Krajewski, Jonathan I. Epstein, John C. Reed, Ki Song, Immunohistochemical analysis of bcl-2, bax, bcl-X, and mcl-1 expression in prostate cancers. American Journal of Pathology. ,vol. 148, pp. 1567- 1576 ,(1996)
Tetsuya Mitsudomi, Hiroyuki Achiwa, Ken-ichi Kozaki, Toyoaki Hida, Hideki Muramatsu, Shigeo Nakamura, Yasushi Yatabe, Takahiko Sugiura, Makoto Ogawa, Takashi Takahashi, Increased Expression of Cyclooxygenase 2 Occurs Frequently in Human Lung Cancers, Specifically in Adenocarcinomas Cancer Research. ,vol. 58, pp. 3761- 3764 ,(1998)
D.A. Jones, D.P. Carlton, T.M. McIntyre, G.A. Zimmerman, S.M. Prescott, Molecular cloning of human prostaglandin endoperoxide synthase type II and demonstration of expression in response to cytokines Journal of Biological Chemistry. ,vol. 268, pp. 9049- 9054 ,(1993) , 10.1016/S0021-9258(18)52976-8
Harri Vainio, Ari Ristimäki, Henrik Wolff, Sisko Anttila, Antti Karjalainen, Kirsi Saukkonen, Expression of cyclooxygenase-2 in human lung carcinoma. Cancer Research. ,vol. 58, pp. 4997- 5001 ,(1998)
Charles E. Eberhart, Robert J. Coffey, Aramandla Radhika, Francis M. Giardiello, Suzanne Ferrenbach, Raymond N. Dubois, Up-regulation of cyclooxygenase 2 gene expression in human colorectal adenomas and adenocarcinomas Gastroenterology. ,vol. 107, pp. 1183- 1188 ,(1994) , 10.1016/0016-5085(94)90246-1
Sgambato A, Tomita N, Yamamoto H, Monden T, Rotterdam H, Weinstein Ib, Yao Y, Cattoretti G, Ciaparrone M, Localization and Expression of p27KIP1 in Multistage Colorectal Carcinogenesis Cancer Research. ,vol. 58, pp. 114- 122 ,(1998)
Ari Ristimäki, Heidi Jänkälä, Pentti Sipponen, Matti Härkönen, Niina Honkanen, Expression of cyclooxygenase-2 in human gastric carcinoma. Cancer Research. ,vol. 57, pp. 1276- 1280 ,(1997)
Denis Labayle, Daniel Fischer, Philippe Vielh, Francois Drouhin, Alex Pariente, Christian Bories, Olivier Duhamel, Michel Trousset, Pierre Attali, Sulindac causes regression of rectal polyps in familial adenomatous polyposis Gastroenterology. ,vol. 101, pp. 635- 639 ,(1991) , 10.1016/0016-5085(91)90519-Q