Overexpression of H-Ryk in mouse fibroblasts confers transforming ability in vitro and in vivo: correlation with up-regulation in epithelial ovarian cancer.
作者： Roger Whittaker , Trivadi S. Ganesan , Mark F. L. Charnock , Sanjiv Manek , Simon Biddolph
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摘要: Abnormalities in the function of receptor tyrosine kinases (RTKs) have been demonstrated to be important pathogenesis cancer. H-Ryk, a new member RTK family, is an unusual that it catalytically inactive because amino acid substitutions conserved residues catalytic domain. We show by immunohistochemistry expressed epithelium, stroma, and blood vessels normal tissues. Evaluation panel 33 primary ovarian tumors (2 benign, 8 borderline, 23 malignant) was performed. H-Ryk overexpressed borderline malignant tumors. In serous clear cell subtypes, there increased expression vessels. Consistent with this observation, overexpression mouse fibroblast line NIH3T3 induces anchorage-independent growth tumorigenicity nude mice. This implies can transforming may therefore significant
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