Bactericidal Activity of Human Macrophages: Analysis of Factors Influencing the Killing of Listeria monocytogenes.

摘要: A technique is described for the measurement of listericidal activity human macrophages grown from blood monocytes. Phagocytosis Listeria monocytogenes was inhibited by a glycolytic poison (NaF) but unaffected anaerobic conditions, cyanide, or 2,4-dinitrophenol (DNP). Killing slower than that neutrophils, and phagocytized began to synthesize deoxyribonucleic acid within 3 hr time ingestion. Differentiated ingested killed more organisms per cell newly isolated Maximal killing required oxygen cyanide DNP. Macrophages patients with chronic intracellular infection (leprosy, tuberculosis, fungal diseases) active Hodgkin's disease were bactericidal normal subjects.