Microdialysis clean-up and sampling in enzyme-based methods for the characterisation of starch.

摘要: Microdialysis was used for sampling enzyme hydrolysis products of starch hydrolysed with beta -amylase, pullulanase, and/or isoamylase, to obtain information about the molecular structure starch. Starches from waxy, normal, and high amylose maize, normal genetically modified potato (amylose deficient) were used, also commercial amyloses. The analysed using high-performance anion-exchange chromatography pulsed amperometric detection (HPAEC-PAD). Simultaneous sample clean-up achieved microdialysis, thus enabling on-line injection into liquid chromatographic system. weight cut-off membrane allowed diffusion small molecules such as oligosaccharides through membrane, but hindered large molecules, e.g. enzymes polysaccharides, entering With microdialysis sampling, it possible investigate short chain fractions debranched in presence without pre-fractionation. microdialysis-HPAEC-PAD system determination A:B ratio P-limit value. After P-amylolysis, only liberated maltose diffused dialysis which resulted branched dextrin well enzyme. proposed method is fast easy handle since hydrolysate (C) 2001 Elsevier Science Ltd. All rights reserved. (Less)