Development of a method for the determination of advanced glycation end products precursors by liquid chromatography and its application in human urine samples
作者： María del Carmen Hurtado-Sánchez , Anunciación Espinosa-Mansilla , María Isabel Rodríguez-Cáceres , Elisabet Martín-Tornero , Isabel Durán-Merás
关键词:
摘要: A liquid chromatographic method with fluorimetric detection has been developed to determine the most abundant α-dicarbonyl compounds, generated as intermediates in Maillard's reaction, previous derivatization high fluorescent pteridinic derivatives. Hence, biomarkers D-glucosone, 3-deoxyglucosone, glyoxal, methylglyoxal, diacetyl, 2,3-pentanedione, and phenylglyoxal were quantified using a gradient elution mode. The experimental conditions of reaction mobile phase composition optimized. Linearity ranges (peak area versus compound concentration) from 1.0 100.0 ng mL(-1) obtained. Detection limits comprised between 0.3 11.0 mL(-1). sensitivity allows determination compounds present human urine, such that are used biomarkers, order investigate their roles several diseases, special emphasis diabetes mellitus. With aim avoiding interferences due cleanup step an ISOLUTE ENV+ cartridge was carried out. concentrations these urinary have reported normalized ratio creatinine, determined healthy diabetic volunteers, different ages sex. In all urine samples, standard addition external calibration procedures applied compared.
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