Response of different genotypes of Brassica carinata to microspore culture

作者: F. Barro , A. Martín

DOI: 10.1046/J.1439-0523.1999.118001079.X

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摘要: Sixteen lines of Brassica carinata were evaluated for microspore culture and plant regeneration. The highest values cell division embryo yield resulted from buds between 2.5 3.5 mm long with 3 days pretreatment at 32°C plating densities 100 000-150 000 microspores/ml. Ten out 16 tested (63%) responded positively to culture. In all breeding F 1 lines, both varied over a wide range, but embryogenic response was higher in than the lines.

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