High-resolution genome-wide mapping of genetic alterations in human glial brain tumors.

摘要: High-resolution genome-wide mapping of exact boundaries chromosomal alterations should facilitate the localization and identification genes involved in gliomagenesis may characterize genetic subgroups glial brain tumors. We have done such using cDNA microarray-based comparative genomic hybridization technology to profile copy number across 42,000 mapped human clones, a series 54 gliomas varying histogenesis tumor grade. This gene-by-gene approach permitted precise sizing critical amplicons deletions detection multiple new aberrations. It has also revealed recurrent patterns occurrence distinct aberrations as well their interrelationships showed that can be clustered into subgroups. A subset detected was shown predominantly associated with either astrocytic or oligodendrocytic phenotype. Finally, five novel minimally deleted regions were identified tumors, containing putative candidate suppressor (TOPORS, FANCG, RAD51, TP53BP1, BIK) could role gliomagenesis.