Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
作者： IF Bambang , YK Lee , DR Richardson , D Zhang , None
DOI: 10.1038/ONC.2012.149
关键词:
摘要: The epithelial-mesenchymal transition (EMT) correlates with disruption of cell-cell adhesion, loss cell polarity and development epithelial malignancy. Identifying novel molecules that inhibit EMT has profound potential for developing mechanism-based therapeutics. We previously demonstrated the endoplasmic reticulum protein 29 (ERp29) is a factor can drive mesenchymal-epithelial (MET) induce growth arrest in MDA-MB-231 cells. Here, we show ERp29 an important molecule establishing integrity during MET. demonstrate regulates MET context-dependent manner. overexpression induced complete mesenchymal cells through downregulating expression transcriptional repressors (for example, Slug, Snai1, ZEB2 Twist) E-cadherin. In contrast, induces incomplete basal-like BT549 which EMT-related markers vimentin; cytokeratin 19 (CK19) E-cadherin) E-cadherin were not altered. However, both cell-types resulted filamentous stress fibers, formation cortical actin restoration phenotype. Mechanistic studies revealed upregulated TJ proteins (zonula-occludens-1 (ZO-1) occludin) core apical-basal (Par3 Scribble) at membrane to enhance contact polarization. Knockdown MCF-7 decreased these proteins, leading adhesion. Taken together, breast cancer
nature.com
sci-hub.se 参考文章(64)
Kevin B. Hoover, Shu-Yuan Liao, Peter J. Bryant, Loss of the tight junction MAGUK ZO-1 in breast cancer: relationship to glandular differentiation and loss of heterozygosity. American Journal of Pathology. ,vol. 153, pp. 1767- 1773 ,(1998) , 10.1016/S0002-9440(10)65691-X
Gérard Joberty, Clark Petersen, Lin Gao, Ian G. Macara, The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42. Nature Cell Biology. ,vol. 2, pp. 531- 539 ,(2000) , 10.1038/35019573
Mirna Perez-Moreno, Colin Jamora, Elaine Fuchs, Sticky Business: Orchestrating Cellular Signals at Adherens Junctions Cell. ,vol. 112, pp. 535- 548 ,(2003) , 10.1016/S0092-8674(03)00108-9
Kazuo Katoh, Yumiko Kano, Mutsuki Amano, Kozo Kaibuchi, Keigi Fujiwara, Stress fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblasts American Journal of Physiology-cell Physiology. ,vol. 280, pp. 1669- 1679 ,(2001) , 10.1152/AJPCELL.2001.280.6.C1669
K Zen, K Yasui, Y Gen, O Dohi, N Wakabayashi, S Mitsufuji, Y Itoh, Y Zen, Y Nakanuma, M Taniwaki, T Okanoue, T Yoshikawa, Defective expression of polarity protein PAR-3 gene (PARD3) in esophageal squamous cell carcinoma. Oncogene. ,vol. 28, pp. 2910- 2918 ,(2009) , 10.1038/ONC.2009.148
Maria J Blanco, Gema Moreno-Bueno, David Sarrio, Annamaria Locascio, Amparo Cano, José Palacios, M Angela Nieto, None, Correlation of Snail expression with histological grade and lymph node status in breast carcinomas. Oncogene. ,vol. 21, pp. 3241- 3246 ,(2002) , 10.1038/SJ.ONC.1205416
Shigeo Ohno, Intercellular junctions and cellular polarity: the PAR–aPKC complex, a conserved core cassette playing fundamental roles in cell polarity Current Opinion in Cell Biology. ,vol. 13, pp. 641- 648 ,(2001) , 10.1016/S0955-0674(00)00264-7
Carien M. Niessen, Tight junctions/adherens junctions: basic structure and function. Journal of Investigative Dermatology. ,vol. 127, pp. 2525- 2532 ,(2007) , 10.1038/SJ.JID.5700865
Daohai Zhang, Thomas C. Putti, Over-expression of ERp29 attenuates doxorubicin-induced cell apoptosis through up-regulation of Hsp27 in breast cancer cells. Experimental Cell Research. ,vol. 316, pp. 3522- 3531 ,(2010) , 10.1016/J.YEXCR.2010.08.014
Luraynne C Sanders, Fumio Matsumura, Gary M Bokoch, Primal De Lanerolle, Inhibition of Myosin Light Chain Kinase by p21-Activated Kinase Science. ,vol. 283, pp. 2083- 2085 ,(1999) , 10.1126/SCIENCE.283.5410.2083