Long-term cultivation of colorectal carcinoma cells with anti-cancer drugs induces drug resistance and telomere elongation: an in vitro study.
作者： Noritsugu Kuranaga , Nariyoshi Shinomiya , Hidetaka Mochizuki
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摘要: The role of telomerase activation in the expression and/or maintenance drug resistance is not clearly understood. Therefore, we investigated relationships, among activity, telomere length and multidrug genes colorectal cancer cell lines cultivated with anti-cancer drugs. LoVo DLD-1 cells were continuously grown presence both CDDP 5-FU for up to 100 days. Cell proliferation, serially monitored as PDL increased. tended increase However, an abnormal aneuploid clone was detected far by a DNA histogram analysis. Tumor showing drugs revealed higher proliferation rate. gradually increased progressive PDL. activity reached maximum level at 15 27 cells. An mRNA components, especially hTERT hTR, observed same PDLs. These results suggest that high elongation telomeres appear help maintain Cancer long proliferative may thus be able better survive exposure This presumably due chromosome stability strong mdr-1 MRP genes.
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