Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products.
作者： Lei He , Mengyu Ren , Xueqing Chen , Xiaoyun Wang , Shan Li
DOI: 10.1007/S00436-014-3935-4
关键词:
摘要: Clonorchis sinensis has been classified as group I biological carcinogen for cholangiocarcinoma by the World Health Organization. Biological studies on excretory/secretory products (ESPs) enabled us to understand pathogenesis mechanism of C. and develop new strategies prevention clonorchiasis. In this study, sequence analysis showed that annexin B30 from (CsANXB30) is composed four repeats which were characterized type II III Ca2+-binding sites or KGD motif with capability Ca2+-binding. addition, immunoblot assay revealed recombinant CsANXB30 (rCsANXB30) could be recognized sera rats infected immunized CsESPs. Real-time PCR its transcriptional level was highest at stage metacercaria. Immunofluorescence employed confirm distributed in tegument, intestine, egg adult worms, well tegument vitellarium rCsANXB30 able bind phospholipid a Ca2+-dependent manner human plasminogen dose-dependent manner. Moreover, cytokine antibody measurements indicated subcutaneously developed strong IL-10 production spleen cells high IgG1 isotype, indicating trigger specific humoral cellular immune response rats. The present results implied might involved host–parasite interaction affected host during infection.
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