Adenosine and 2-phenylaminoadenosine (CV-1808) inhibit human neutrophil bactericidal function.

摘要: Adenosine is a natural autocoid and immunomodulator that serves an anti-inflammatory role. Stimulation of polymorphonuclear neutrophils (PMN) with soluble stimuli has been shown to inhibit the PMN oxidative burst. We examined effects adenosine analog 2-phenylaminoadenosine (CV-1808) on bactericidal function. (10 mM) CV-1808 100 microM) inhibited killing Staphylococcus aureus. There were more surviving bacteria after 240 min incubation S. aureus or (100 (254% +/- 45% 739% 88% control, respectively) (P less than 0.05) there in control. In contrast, inosine mM), major degradation product adenosine, did not affect killing. alter cell association aureus, but aureus-activated superoxide release was decreased by 67% 7% 32% 12% respectively 0.05). Since function only at approximately 10,000 times peak physiological concentrations, endogenous levels would be expected adversely On other hand, pharmacological concentrations derivatives may decrease burst sufficiently increase host susceptibility infection.