Volatile Markers of Breast Cancer in the Breath

摘要: Breast cancer is accompanied by increased oxidative stress and induction of polymorphic cytochrome P-450 mixed oxidase enzymes (CYP). Both processes affect the abundance volatile organic compounds (VOCs) in breath because causes lipid peroxidation polyunsaturated fatty acids membranes, producing alkanes methylalkanes which are catabolized CYP. We performed a pilot study VOCs, potential new marker disease women with breast cancer. This was combined case-control cross-sectional abnormal mammograms scheduled for biopsy. Breath samples were analyzed gas chromatography mass spectroscopy order to determine methylated alkane contour (BMAC), three-dimensional display alveolar gradients (abundance minus room air) C4-C20 monomethylated alkanes. BMACs without compared using forward stepwise discriminant analysis. Two hundred one obtained from biopsies read two pathologists. There 51 cases 198 concordant biopsies. The test distinguished between healthy volunteers sensitivity 94.1% (48/51) specificity 73.8% (31/42) (cross-validated 88.2% (45/51), (31/42)). Compared no on biopsy, identified 62.7% (32/51) 84.0% (42/50) 60.8% (31/51), 82.0% (41/50)). negative predictive value (NPV) screening superior mammogram (99.93% versus 99.89%); positive (PPV) (4.63% 1.29%). A markers accurately cancer, an NPV mammogram. could potentially be employed as primary screen Confirmatory studies larger groups required.