Detection of Helicobacter pylori infection in patients with obstructive airway diseases with sero techniques using highly specific IgG antibodies for Helicobacter pylori antigen
作者： Subhash Pawar , Sanditi Ram Reddy , Lakshmi Kiran Chelluri , Chelluri Eswara Prasad
DOI: 10.1016/S2222-1808(14)60472-2
关键词:
摘要: Abstract Objective To determine the authenticity of three techniques viz. , ELISA, western blot and indirect immune fluorescence assay (IIFA) to establish connection between Helicobacter pylori ( H. ) two obstructive airway diseases, chronic pulmonary diseases (COPD) asthma. Methods Serum samples were collected from 48 patients, 27 with COPD, 21 asthma control sera obtained 42 healthy volunteer blood donors. analyzed by sero-based techniques, viz ., IIFA. Results ELISA results revealed no infection COPD Western also did not reveal any relationship diseases. Antibody pattern support these IIFA tests a positive connectivity relation Conclusions The present investigations an association in is reliable test hence it recommended.
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