Differential contribution of direct-developing and stem cell-derived melanocytes to the zebrafish larval pigment pattern

摘要: Abstract The extent of adult stem cell involvement in embryonic growth is often unclear, as reliable markers or assays for whether a derived from an cell, such the melanocyte (MSC), are typically not available. We have previously shown that two lineages melanocytes can contribute to larval zebrafish pigment pattern. embryo first develops ontogenetic pattern largely composed ErbB-independent, direct-developing melanocytes. This population be replaced during regeneration by ErbB-dependent MSC-derived following ablation. In this study, we developed differentiation assay used together with drugs ablate MSC investigate found essentially all develop before 3 dpf arise population. Similarly, late-developing (after 3 dpf) head also ErbB independent. contrast, after 3 days postfertilization lateral and dorsal stripe sensitive inhibitor, indicating they MSC. show mutants kitj1e99 skiv2l2j24e1 grossly normal overall lack contribution stripe. result suggests underlying defect these mutations regulation. suggest regulative functions may serve quality control roles development, addition its established homeostasis adult.