Clarithromycin resistance in Helicobacter pylori and its molecular determinants in Northern Spain, 2013-2015.
作者： Esther Tamayo , Milagrosa Montes , María Fernández-Reyes , Jacobo Lizasoain , Begoña Ibarra
DOI: 10.1016/J.JGAR.2016.12.019
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摘要: Abstract Objectives Clarithromycin resistance (CLR-R) is the main reason for failure of Helicobacter pylori infection treatment, which frequently empirically prescribed due to erroneous belief that culture susceptibility testing difficult. The aim this study was determine CLR-R in a region southern Europe and evaluate utility PCR sequencing assay applied on gastroduodenal biopsies detecting H. clarithromycin (CLR) susceptibility. Methods all isolates obtained by during 2013–2015 determined Etest. During 2014–2015, detection CLR were also studied followed performed biopsies. Point mutations 23S rRNA gene CLR-resistant 2014. Results Of 1986 (63 from children 1923 adults), 349 (17.6%) [21/63 (33.3%) 328/1923 (17.1%) adults; P 0.001], 31.5% resistant levofloxacin. detected A2147G (79.8%), A2146G (17.2%) A2146C (2%). Concordance between Etest after 89.8%. Conclusions high Gipuzkoa, northern Spain. molecular method directly good alternative traditional an aid when non-viable.
sci-hub.se 参考文章(19)
A. Cosme, M. Montes, M. Martos, I. Gil, U. Mendarte, Y. Salicio, L. Piñeiro, M.T. Recasens, B. Ibarra, C. Sarasqueta, L. Bujanda, Usefulness of antimicrobial susceptibility in the eradication of Helicobacter pylori Clinical Microbiology and Infection. ,vol. 19, pp. 379- 383 ,(2013) , 10.1111/J.1469-0691.2012.03844.X
J Versalovic, D Shortridge, K Kibler, M V Griffy, J Beyer, R K Flamm, S K Tanaka, D Y Graham, M F Go, Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori. Antimicrobial Agents and Chemotherapy. ,vol. 40, pp. 477- 480 ,(1996) , 10.1128/AAC.40.2.477
Angel Cosme, Jacobo Lizasoan, Milagrosa Montes, Esther Tamayo, Horacio Alonso, Usua Mendarte, Maider Martos, María Fernández-Reyes, Cristina Saraqueta, Luis Bujanda, Antimicrobial Susceptibility-Guided Therapy Versus Empirical Concomitant Therapy for Eradication ofHelicobacter pyloriin a Region with High Rate of Clarithromycin Resistance Helicobacter. ,vol. 21, pp. 29- 34 ,(2016) , 10.1111/HEL.12231
Milagrosa Montes, Flor N. Villalon, Francisco J. Eizaguirre, Maider Delgado, Ignacio M. Muñoz-Seca, María Fernández-Reyes, Emilio Pérez-Trallero, Helicobacter pyloriInfection in Children. Antimicrobial Resistance and Treatment Response Helicobacter. ,vol. 20, pp. 169- 175 ,(2015) , 10.1111/HEL.12187
Michael Selgrad, Julia Meile, Jan Bornschein, Arne Kandulski, Cosima Langner, Mariya Varbanova, Thomas Wex, Ina Tammer, Dirk Schlüter, Peter Malfertheiner, Antibiotic susceptibility of Helicobacter pylori in central Germany and its relationship with the number of eradication therapies. European Journal of Gastroenterology & Hepatology. ,vol. 25, pp. 1257- 1260 ,(2013) , 10.1097/MEG.0B013E3283643491
Nicolas Kalach, Pierre Gosset, Eric Dehecq, Anne Decoster, Claire Spyckerelle, Stephan Papadopolos, Christophe Dupont, Josette Raymond, Usefulness of Gastric Biopsy–Based Real-Time Polymerase Chain Reaction for the Diagnosis of Helicobacter pylori Infection in Children Journal of Pediatric Gastroenterology and Nutrition. ,vol. 61, pp. 307- 312 ,(2015) , 10.1097/MPG.0000000000000787
Yuan Wenzhen, Li Yumin, Guan Quanlin, Yang Kehu, Jiang Lei, Wang Donghai, Yang Lijuan, Is Antimicrobial Susceptibility Testing Necessary Before First-line Treatment for Helicobacter pylori Infection? -Meta-analysis of Randomized Controlled Trials- Internal Medicine. ,vol. 49, pp. 1103- 1109 ,(2010) , 10.2169/INTERNALMEDICINE.49.3031
Mitsushige Sugimoto, Takahiro Uotani, Shu Sahara, Hitomi Ichikawa, Mihoko Yamade, Ken Sugimoto, Takahisa Furuta, Efficacy of tailored Helicobacter pylori eradication treatment based on clarithromycin susceptibility and maintenance of acid secretion. Helicobacter. ,vol. 19, pp. 312- 318 ,(2014) , 10.1111/HEL.12128
Alba A. Trespalacios, Emiko Rimbara, William Otero, Rita Reddy, David Y. Graham, Improved allele-specific PCR assays for detection of clarithromycin and fluoroquinolone resistant of Helicobacter pylori in gastric biopsies: identification of N87I mutation in GyrA Diagnostic Microbiology and Infectious Disease. ,vol. 81, pp. 251- 255 ,(2015) , 10.1016/J.DIAGMICROBIO.2014.12.003
Armelle Ménard, Adriana Santos, Francis Mégraud, Mónica Oleastro, PCR-Restriction Fragment Length Polymorphism Can Also Detect Point Mutation A2142C in the 23S rRNA Gene, Associated with Helicobacter pylori Resistance to Clarithromycin Antimicrobial Agents and Chemotherapy. ,vol. 46, pp. 1156- 1157 ,(2002) , 10.1128/AAC.46.4.1156-1157.2002