Restricted access of proteins to mannan polysaccharides in intact plant cell walls

摘要: How the diverse polysaccharides present in plant cell walls are assembled and interlinked into functional composites is not known detail. Here, using two novel monoclonal antibodies a carbohydrate-binding module directed against mannan group of hemicellulose wall polysaccharides, we show that molecular recognition intact severely restricted. In secondary walls, esterification can prevent probe epitopes/ligands, detection mannans primary be effectively blocked by presence pectic homogalacturonan. Masking homogalacturonan shown to widespread phenomenon parenchyma systems, masked was found feature regions at pit fields. Direct fluorescence imaging mannan-specific sequential enzyme treatments with an endo-β-mannanase confirmed cryptic epitopes masking pectin potential mechanism for controlling micro-environments.