Microphotometric and autoradiographic studies on the cell cycle and cell size during growth and decline of Chinese hamster cell cultures.

摘要: Summary A combination of (1) microphotometric determinations the DNA and protein content dry mass single cells (2) autoradiography was used to follow changes in cell cycle parameters growth cultures Chinese hamster as they passed from initial stages growth, through exponential phase, into decline. Cell counts total were overall culture growth. In lag phase early part size relative duration combined periods S M increased. latter half at mitosis decreased, while length initiation synthesis remained essentially constant. As went decline resulting death, increased greatly, primarily because an increase G1. At thesame time, continued go with very little net content. There evidence that least 40% well period, no any left thecycle. description events can be given terms rates three processes: synthesis, mitosis. are indications rate is less closely associated than synthesis.