Basement Membrane Mimics of Biofunctionalized Nanofibers for a Bipolar-Cultured Human Primary Alveolar-Capillary Barrier Model.

摘要: In vitro reconstruction of an alveolar barrier for modeling normal lung functions and pathological events serve as reproducible, high-throughput pharmaceutical platforms drug discovery, diagnosis, regenerative medicine. Despite much effort, the organ-level has failed due to lack structural similarity natural basement membrane, functionalization with specific ligands cell function, use primary cells biodegradability. Here we report a bipolar cultured alveolar-capillary model human supported by membrane mimics fully synthetic bifunctional nanofibers. One-step electrospinning process using bioresorbable polyester multifunctional star-shaped polyethylene glycols (sPEG) enables fabrication ultrathin nanofiber mesh interconnected pores. The possessed mechanical stability against cyclic expansion seen in vivo. sPEGs additive...