High-molecular-weight melanoma-associated antigen mimotope immunizations induce antibodies recognizing melanoma cells.

摘要: Size and posttranslational modifications are obstacles in the recombinant expression of high-molecular-weight melanoma-associated antigen (HMW-MAA). Creating a tumor mimic via phage display technology may be means to overcome this problem for vaccine design. In study, we aimed generate an immunogenic epitope HMW-MAA. Therefore screened linear 9mer peptide library, using anti-HMW-MAA monoclonal antibody (mAb) 225.28S. This mediates antibody-dependent cellular cytotoxicity (ADCC) has already been used anti-idiotype therapy trials. Fifteen peptides were selected by mAb 225.28S biopanning procedure. They share consensus sequence, but show only partial homology amino acid sequence HMW-MAA core protein, indicating mimicry with conformational epitope. One mimotope was chosen fused albumin binding protein (ABP) as carrier. Immunoassays indicated that fusion folded correctly. Subsequently, tested immunogenicity BALB/c mice. The induced anti-mimotope antibodies recognized 518A2 human melanoma cells, whereas sera mice immunized carrier ABP alone showed no reactivity. These capable inducing specific lysis cells ADCC assays murine effector cells. conclusion, presented data indicate mimotopes suitable tools elicit epitope-specific anti-melanoma immune responses.