The Murine mCLCA3 (Alias gob-5) Protein Is Located in the Mucin Granule Membranes of Intestinal, Respiratory, and Uterine Goblet Cells
作者： Ina Leverkoehne , Achim D. Gruber
DOI: 10.1177/002215540205000609
关键词:
摘要: The putative anion channel mCLCA3 (alias gob-5) is the third murine member of recently discovered family calcium-activated chloride channels (CLCA family). Preliminary data suggest that may play a significant role in diseases with secretory dysfunctions, including asthma and cystic fibrosis. In this study, protein was characterized biochemically its cellular subcellular distribution pattern established normal tissues. Polyclonal rabbit antibodies were generated affinity-immunopurified using synthetic oligopeptides corresponding to extracellular amino terminus polypeptide. After vitro translation glycosylation, proteinase K protection assay, heterologous expression COS-7 or HEK 293 cells, SDS-PAGE immunoblotting revealed structure similar previously CLCA proteins. A systematic light, confocal laser scanning, transmission electron microscopic immunolocalization virtually all tissues, identified exclusively associated mucin granule membranes gastrointestinal, respiratory, uterine goblet cells other mucin-producing cells. results be involved synthesis, condensation, secretion mucins.
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sci-hub.se 参考文章(37)
Magali Agnel, Thierry Vermat, Jean-Michel Culouscou, Identification of three novel members of the calcium‐dependent chloride channel (CaCC) family predominantly expressed in the digestive tract and trachea1 FEBS Letters. ,vol. 455, pp. 295- 301 ,(1999) , 10.1016/S0014-5793(99)00891-1
S Ran, D.J. Benos, Immunopurification and structural analysis of a putative epithelial Cl- channel protein isolated from bovine trachea. Journal of Biological Chemistry. ,vol. 267, pp. 3618- 3625 ,(1992) , 10.1016/S0021-9258(19)50569-5
S Ran, D J Benos, Isolation and functional reconstitution of a 38-kDa chloride channel protein from bovine tracheal membranes. Journal of Biological Chemistry. ,vol. 266, pp. 4782- 4788 ,(1991) , 10.1016/S0021-9258(19)67717-3
Achim D. Gruber, Renu Gandhi, B. U. Pauli, The murine calcium-sensitive chloride channel (mCaCC) is widely expressed in secretory epithelia and in other select tissues Histochemistry and Cell Biology. ,vol. 110, pp. 43- 49 ,(1998) , 10.1007/S004180050263
A. Gruber, C. Fuller, R. Elble, D. Benos, B. Pauli, The CLCA Gene Family A Novel Family of Putative Chloride Channels Current Genomics. ,vol. 1, pp. 201- 222 ,(2000) , 10.2174/1389202003351526
Pamela C. Colony, Structural characterization of colonic cell types and correlation with specific functions. Digestive Diseases and Sciences. ,vol. 41, pp. 88- 104 ,(1996) , 10.1007/BF02208589
Achim D. Gruber, Bendicht U. Pauli, Molecular cloning and biochemical characterization of a truncated, secreted member of the human family of Ca2+-activated Cl- channels. Biochimica et Biophysica Acta. ,vol. 1444, pp. 418- 423 ,(1999) , 10.1016/S0167-4781(99)00008-1
A.T. Hoogeveen, J. Keulemans, R. Willemsen, B.J. Scholte, J. Bijman, M.J. Edixhoven, H.R. De Jonge, H. Galjaard, Immunological localization of cystic fibrosis candidate gene products. Experimental Cell Research. ,vol. 193, pp. 435- 437 ,(1991) , 10.1016/0014-4827(91)90118-E
L. Romio, L. Musante, R. Cinti, M. Seri, O. Moran, O. Zegarra-Moran, L.J.V. Galietta, Characterization of a murine gene homologous to the bovine CaCC chloride channel. Gene. ,vol. 228, pp. 181- 188 ,(1999) , 10.1016/S0378-1119(98)00620-9
Jerold A Last, Ralph C Frates, Tokio T Kaizu, Mucus glycoproteins secreted by respiratory epithelial tissue from cystic fibrosis patients Pediatric Research. ,vol. 17, pp. 30- 34 ,(1983) , 10.1203/00006450-198301000-00006