Continuous epidermal growth factor delivery in corneal epithelial wound healing.
作者： D S Rootman , H Sheardown , C Wedge , L Chou , Y L Cheng
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摘要: PURPOSE: To investigate the effects of a single prolonged exposure to recombinant epidermal growth factor on healing anterior keratotomy wounds in New Zealand white rabbits. METHODS: After wounding, eyes were perfused for 1, 2, 4, and 8 hours with either solution at concentration 50 micrograms/ml or balanced saline using Morgan therapeutic lens (Mortan Inc, Missoula MT) syringe pump. Furthermore, response was evaluated by perfusing solutions concentrations 5, 50, 100 500 4 hours. Wound rates determined quantitative morphometry wound area. The ratio control provided measure effect healing, defined as enhancement factor. RESULTS: found be 1.04 +/- 0.08, 1.17 0.07, 1.43 0.09, 1.59 0.07 perfusion times hours, respectively. factors 0.99 1.21 0.95 100, 4-hour perfusions, CONCLUSION: results indicated that continuous exposures few 2 produced significant increase (P < 0.05); increasing time further increases rate healing. Results from experiments showed optimum enhancing epithelial is approximately micrograms/ml.
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