Oncogenic activation of MAP kinase by BRAF pseudogene in thyroid tumors.

摘要: Activating BRAF mutations have been reported in 40% of papillary thyroid carcinomas (PTCs). The involvement pseudogene tumorigenesis has not previously studied. We investigated expression 68 tumors: 16 multinodular goiters, 43 classic PTCs, 6 follicular variants PTC, and 3 anaplastic carcinomas. function was studied by Western blots, soft agar assay, nude mice. detected 7 18 1 PTC. There is an inverse correlation between mutation. transcripts were more frequently tumors without mutation than those with Furthermore, could activate the MAP kinase signaling pathway, transform NIH3T3 cells vitro, induce These data suggest that activation may play a role tumor development.