Advances in genetic circuit design: novel biochemistries, deep part mining, and precision gene expression.

摘要: Cells use regulatory networks to perform computational operations respond their environment. Reliably manipulating such would be valuable for many applications in biotechnology; example, having genes turn on only under a defined set of conditions or implementing dynamic temporal control expression. Still, building synthetic circuits remains one the most difficult challenges genetic engineering and as result they have not found widespread application. Here, we review recent advances that address key forward design circuits. First, look at new concepts, including construction layered digital analog circuits, approaches circuit response functions. Second, work apply part mining expand number regulators can used together within cell. Finally, describe obtain precise gene expression reduce context dependence will accelerate by more reliably balancing while reducing toxicity.