MicroRNA-26a inhibits proliferation by targeting high mobility group AT-hook 1 in breast cancer.

摘要: Objectives: To investigate the crucial role of miR-26a in breast cancer and to validate whether could regulate proliferation cells by targeting high mobility group AT-hook 1 (HMGA1). Methods: Reverse transcription-polymerase chain reaction (RT-PCR) was used quantify expression levels adjacent non-cancerous tissues. MTT, cell migration invasion assay were carried out characterize function. Finally, target gene miR-26a, luciferase reporter employed, followed RT-PCR Western blot confirmation. Results: Compared with normal tissues, a significant down-regulation observed tissues (P=0.002). suppresses MDA-MB-231 Mcf-7 lines motility. The activity significantly decreased after co-transfection psiCHECK-2/HMGA1 3’-UTR mimics comparison control cells, qRT-PCR blotting analysis found that HMGA1 at mRNA protein mimic-treatment relative NC. MTT showed down regulation siRNA enhance tumor-suppressive effect (P < 0.05). Conclusions: results present study indicate may be associated human carcinogenesis, which inhibits tumor HMGA1.