Malignant ascites-derived exosomes promote proliferation and induce carcinoma-associated fibroblasts transition in peritoneal mesothelial cells.
作者： Mingtian Wei , Tinghan Yang , Xiangzheng Chen , Yangping Wu , Xiangbing Deng
DOI: 10.18632/ONCOTARGET.15040
关键词:
摘要: Malignant ascites-derived exosomes have been demonstrated to participate in tumor metastasis. In peritoneal metastasis, normal mesothelial cells (MCs) can be converted into carcinoma-associated fibroblasts (CAFs) by mesothelial-mesenchymal transition (MMT). Herein, we evaluated the effect of malignant on MCs vitro and vivo experiments determine whether could educate contribute metastasis.Under treatment exosomes, showed increased ability proliferate migrate. Expression CAFs specific proteins markers MCs, including fibroblast activation protein (FAP), alpha-smooth muscle actin (α-SMA), fibronectin, were after exosomes. clinical samples test, TGF-β1 was found overexpressed both ascites high volume may responsible for peritoneum fibrosis. addition, increase xenograft growth suppressing inhibitive MCs. Besides, FAP, α-SMA, vimentin biopsies. The immunohistochemical staining mice biopsies also revealed expression fibronectin along with decreased E-cadherin VCAM-1 treatment.Thus, importance development metastasis facilitating convert induced MMT.
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