Purification of fetal hematopoietic progenitors and demonstration of recombinant multipotential colony-stimulating activity.

摘要: To facilitate the direct study of progenitor cell biology, we have developed a simple and efficient procedure based upon negative selection by panning to purify large numbers committed erythroid myeloid progenitors from human fetal liver. The nonadherent, panned cells constitute highly enriched population cells, containing 30.4 +/- 13.1% erythrocyte burst forming units (BFU-E), 5.5 1.9% granulocyte-macrophage colony (CFU-GM), 1.4 0.7% granulocyte-erythroid-macrophage-megakaryocyte (CFU-GEMM) as assayed in methylcellulose cultures. These are morphologically immature blasts with prominent Golgi. This preparative method recovers 60-100% detectable unfractionated liver yields 2-30 X 10(6) each sample, thus provides sufficient allow biochemical immunologic manipulation. Using this technique, purified recombinant protein previously thought only stimulating activity (GM-CSA) is shown both promoting multipotential activity. Progenitor purification appears be simple, that should hematopoietic their differentiation.