Analysis of metal-induced DNA lesions and DNA-repair replication in mammalian cells.

摘要: The potency of several metal compounds in causing lesions DNA either directly or by exposure intact cultured cells has been examined using the neutral conditions nucleoid gradient sedimentation. HgCl2 was clearly most potent inducer single-strand breakage when added to isolated nucleoids were prepared from treated with this compound. CaCrO4 , however, caused DNA-strand breaks agent but did not induce strand nucleoids. Although less than HgCl2, NiCl2 also significant single metal. Since may occur secondary activation DNA-dependent nucleases during repair replication, CsCl density sedimentation utilized examine whether processes induced NiCl2, and . substantial DNA-repair activity at concentrations times where could be detected whereas a 10-fold lower level compared optimal which again below that produced measurable lesions. Both induction these metals concentration- time-dependent. These results demonstrate some unique aspects interaction point possible important correlation carcinogenesis since nickel chromate have implicated as carcinogens considerable is considered carcinogen induces least despite its producing