The use of HRMAS NMR spectroscopy to study the in vivo intra–cellular carbon/nitrogen ratio of Solieria chordalis (Rhodophyta)

摘要: The red alga Solieria chordalis (J. Agardh) C. Agardh (Rhodophyta) was used as a model to investigate the effects of changes in seawater salinity on carbon/nitrogen ratio. Carbohydrates and nitrogenous metabolites are major components this their metabolisms intimately linked. Previous publications have provided solid database for these two primary metabolic pathways from experiments observations situ. Storage products (e.g. floridean starch), cell wall polysaccharides (carrageenan) low molecular weight carbohydrates such floridoside digeneaside compounds constituting pool available carbon. Compounds amino acids peptides, constitute nitrogen. This study focuses intracellular C/N ratio inside compounds. can be defined balance between acids. High–resolution magic angle spinning nuclear magnetic resonance spectroscopy (HRMAS NMR) provides powerful approach vivo analysis organic These results were complimented with quantitative data obtained high performance liquid chromatography (HPLC). In vitro experimental analyses order effect salt stress ratio, S. cultured under controlled conditions. Effects hyposalinity hypersalinity stresses (low 22 ‰ 50 salinity) tested. Both HPLC NMR data, stressed unstressed algae, generated insights into variations carbonated metabolites, involving ratios, demonstrated adaptive responses seaweed.