McDonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms).

摘要: The genetic structure of the McDonough strain feline sarcoma virus (SM-FeSV) was deduced by analysis molecularly cloned, transforming proviral DNA. 8.2-kilobase pair SM-FeSV provirus is longer than those other viruses and contains a gene (v-fms) flanked sequences derived from leukemia virus. order genes with respect to viral RNA 59-gag-fms-env-39, in which entire env an almost complete gag sequence are represented. Transfection NIH/3T3 cells cloned DNA induced foci morphologically transformed expressed products contained rescuable genomes. Cells infection or after transfection polyprotein (P170gag-fms) characteristic strain. Two proteolytic cleavage (P120fms pp55gag) were also found immunoprecipitates metabolically labeled, cells. An additional polypeptide, detected at comparatively low levels transformants, indistinguishable size antigenicity envelope precursor (gPr85env) complexity v-fms (3.1 +/- 0.3 kilobase pairs) approximately twofold greater oncogene (v-fes) Snyder-Theilen Gardner-Arnstein FeSV. By heteroduplex, restriction enzyme, nucleic acid hybridization analyses, v-fes showed no detectable homology one another. Radiolabeled fragments representing portions two oncogenes hybridized different EcoRI HindIII normal cat cellular Cellular related (designated c-fms) much more complex c-fes distributed segmentally over 40 pairs Comparative structural studies proviruses Synder-Theilen, Gardner-Arnstein, that region feline-leukemia genome pol-env junction represented adjacent v-onc each FeSV may have provided preferred for recombination genes. Images