17β-Estradiol Restores Endothelial Nitric Oxide Release to Shear Stress in Arterioles of Male Hypertensive Rats

摘要: Background—Endothelial nitric oxide (NO)–mediated responses are impaired in arterioles of male spontaneously hypertensive rats (SHR), but they still present female SHR. We hypothesized that vitro incubation SHR with estrogen will restore NO-mediated by upregulation endothelial NO synthase. Methods and Results—Responses to increases perfusate flow (from 0 25 μL/min) the calcium ionophore A23187 (5×10−8 10−6 mol/L), norepinephrine (NE; 10−7 3×10−7 sodium nitroprusside (SNP; 10−8 adenosine (ADO; 5×10−5 mol/L) were studied cannulated pressurized gracilis muscle (≈75 μm diameter) isolated from 12-week-old before after 10−9 mol/L 17β-estradiol (17β-E2) for 16 18 hours. After 17β-E2, basal diameter was significantly increased (by ≈10%), flow-induced dilation enhanced (79.8±2.9 versus 103.7±3.7 at μL/min),...