Delineating melanoma using multimodal polarized light imaging.

摘要: Background and Significance Melanoma accounts for 3% of all skin cancers but causes 83% cancer deaths. The first step in treatment melanoma is the removal lesions, usually by surgical excision. Currently most lesions are removed without intraoperative margin control. Post-operative methods inspect 1–2% prone to sampling errors. In this study we evaluate use reflectance fluorescence polarization imaging demarcation thick fresh excisions. Materials Methods Pigmented clinically suspicious were elliptically excised with proper margins. Elliptical excisions vertically bisected along short axis specimen into two halves middle pigmented lesions. tumor face was imaged. After that, one half sample briefly stained aqueous 2 mg/ml solution tetracycline, whereas another 0.2 methylene blue. Then both specimens reimaged. Reflectance images acquired spectral range between 390 750 nm. Fluorescence tetracycline-stained tissue excited at nm registered 450 700 blue-stained samples 630 650 imaging, processed standard H&E histopathology. resulting histological optical compared each other. Results Conclusions Our findings demonstrate that tetracycline blue suitable dysplastic benign nevi. Melanoma better delineated Accurate rapid delineation appears feasible. Lasers Surg. Med. 41:10–16, 2009. © 2008 Wiley-Liss, Inc.