HPTLC Analysis, Antioxidant and Antigout Activity of Indian Plants.
作者： Shivraj Hariram Nile , Se Won Park
关键词:
摘要: The HPTLC analysis, antioxidant, and antigout activity of Asparagus racemosus, Withania somnifera, Vitex negundo, Plumbago zeylanica, Butea monosperma Tephrosia purpurea extracts were investigated. chemical fingerprinting carried out by high performance thin layer chromatography (HPTLC), antioxidant ABTS, DPPH, FRAP radical scavenging assays, antiogout cow milk xanthine oxidase. fingerprint qualitatively revealed predominant amount flavonoids. TEAC values ranged from 45.80 to 140 µM trolox/100 g dry weight for 85 430 trolox/ 100 dw 185 560 respectively. Plants used in this study was found inhibit the toxicity, as seen decreased LPO increased GSH, SOD CAT levels. total phenolic flavonoid content 10.21 28.17 5.80 10.1 mg gallic acid equivalents (GAE)/100 gdw plant demonstrated significant oxidase inhibitory at g/mL an inhibition greater than 50 % IC50 below standard. This effect almost similar allopurinol (Standard drug) against (90.2 ± 0.4 %). These root extract will be subjected further extensive studies isolate identify their active constituents which are useful inflammation gout.
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