Role of a specific endogenous fatty acid fraction in the coupling-uncoupling mechanism of oxidative phosphorylation of brown adipose tissue.
作者： A. Bulychev , R. Kramar , Z. Drahota , Olov Lindberg
DOI: 10.1016/0014-4827(72)90579-4
关键词:
摘要: Abstract Sucrose isolated brown adipose tissue mitochondria are uncoupled. They contain between 120–150 nEq of free fatty acids per mg protein. The removal by means carnitine plus ATP a minor fraction these bound FFA transfers the from an uncoupled to coupled state. If very last traces this removed resume all characteristics e.g. respiratory control, ATP-Pi exchange and DNP-stimulated ATPase lack endogenous Mg-requiring ATPase. Some system also altered. changes in many biochemical parameters paralleled ultrastructure mitochondria. nature freshly is that DNP- not detergent-induced mechanism which mitochondrial exerts its action on different DNP. differences observed parameters, including ultrastructure, native recoupled mitochondria, suggest effect allosteric nature. physiologic role described discussed.
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