Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival

摘要: Cervical cancer is typically well infiltrated by immune cells. Because of the intricate relationship between cells and cells, we aimed to identify both cell expressed biomarkers. Using a novel approach, isolated RNA from flow-sorted viable EpCAM+ tumor epithelial CD45+ tumor-infiltrating obtained squamous cervical samples (n = 24). Total was sequenced differential gene expression analysis fractions identified TCL1A as marker for predicting improved survival (p 0.007). This finding validated using qRT-PCR 0.005) partially immunohistochemistry 0.083). Importantly, found be in subpopulation B (CD3-/CD19+/CD10+/CD34-) multicolor immunofluorescence. A high TCL1A/CD20 (B cell) ratio, determined total separate patient cohort 52), also correlated with 0.027). first study demonstrating prognostic value separating determining their profile identifying putative Our results suggest that intratumoral TCL1A+ are important controlling development.