Suppressing the malignant phenotypes of glioma cells by lentiviral delivery of small hairpin RNA targeting hypoxia-inducible factor-1α
作者： Jun Yu , William K K Wu , Su Yin Feng , Jun Fei Shao , Chang Gui Dong
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摘要: Hypoxic microenvironment of solid tumors is known to shape malignant phenotypes cancer cells through the dimeric transcription factor hypoxia-inducible (HIF)-1. In present study, therapeutic effect targeting α subunit HIF-1 in glioma via lentiviral delivery small hairpin RNA (shRNA) was evaluated. Data from quantitative real-time PCR and immunohistochemistry demonstrated that HIF-1α progressively upregulated during development gliomas. Lentiviral shRNA led substantial loss cell viability, G0/G1-phase cycle arrest, apoptosis, impairment motility invasiveness human U87MG cells. Xenograft experiments nude mice further showed HIF-1α-shRNA inhibited tumor growth caused persistent repression its target genes, including VEGF, GLUT1 MMP2, up 25 days post-inoculation. Taken together, a promising approach for glioma.
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