Alpha melanocyte stimulating hormone (α-MSH) enhances eicosanoid production by bovine retinal pigment epithelium

摘要: Abstract Explants of bovine eyes consisting retina, with its underlying choroid and sclera (termed retinal explants) were maintained in organ culture the absence or presence α -melanocyte stimulating hormone ( -MSH) for up to 19 days. The conditioned media was collected twice a week assayed following eicosanoids, prostaglandin E 2 (PGE ) prostacyclin. addition -MSH incubation resulted 1.5 fold enhancement production both PGE This stimulatory effect diminished after 11 Additionally, three tissue components comprising explants i.e. 1. neural retina 2. pigment epithelium (RPE) vascular layer (choroid) 3. scleral separated incubated -MSH. Hormone treatment caused an enhanced eicosanoid by RPE alone, while neuronal reduced unaffected respectively. demonstrates that is source induced observed whole explant. Our findings demonstrate, first time can stimulate culture.